Table 1. Bacterial strains and plasmids used in study.
| Bacterial strains and plasmids | Characteristics | Reference |
|---|---|---|
| Ralstonia solanacearum | ||
| SL341 | Wild-type, isolated from tomato plants, Race 1, Biovar4 | 34 |
| SL341D | hppD (RSc3103):: Tn5; Kanr | This study |
| SL341DC | Trans conjugant of SL341Dcarrying pRKD, complementation of SL341D; Kanr, Tcr | This study |
| SL341S | rpoS (RSc1207):: Tn5; Kanr | This study |
| SL341SC | Trans conjugant of SL341Scarrying pRKS, complementation of SL341S; Kanr, Tcr | This study |
| SL341R | oxyR (RSc2690):: Tn5; Kanr | This study |
| SL341RC | Trans conjugant of SL341Rcarrying pRKR, complementation of SL341R; Kanr, Tcr | This study |
| SL341G | hrpG (RSp0852):: Tn5; Kanr | This study |
| SL341GC | Trans conjugant of SL341G carrying pRKG, complementation of SL341G; Kanr, Tcr | This study |
| Escherichia coli | ||
| DH5α | F-ɸ80lacZDM15 D(lacZYA-argF)U169 deoRrecA1 endA1 hsdR17 (rk-,mk+) phoA supE44λ- thi-1 gyrA96 relA1 | 39 |
| HB101 | F-thi-1hsdS20 (rB-, mB-) supE44 recA13 ara-14 leuB6 proA2 lacY1 galK2 rpsL20 (strr) xyl-5 mtl-1 | 41 |
| Plasmid | ||
| pUC119 | Apr; cloning vector | 38 |
| pGEM-T Easy | Apr; T/A cloning vector | Promega |
| pGEMD | Apr; pGEM-T Easy carrying 1.34 kb fragment of hppD gene of R. solanacearum SL341 | This study |
| pGEMS | Apr; pGEM-T Easy carrying 1.53 kb fragment of rpoS gene of R. solanacearum SL341 | This study |
| pGEMR | Apr; pGEM-T Easy carrying 1.24 kb fragment of oxyR gene of R. solanacearum SL341 | This study |
| pGEMG | Apr; pGEM-T Easy carrying 1.14 kb fragment of hrpG gene of R.solanacearum SL341 | This study |
| pRK415 | Tcr; PK2-derived broad host range cloning vector | 40 |
| pRKD | Tcr; pRK415 carrying 1.36 kb EcoR1 fragment of pGEMD containing hppD gene of R. solanacearum SL341 | This study |
| pRKS | Tcr; pRK415 carry 1.55 kb EcoR1 fragment of pGEMS containing rpoS gene of R. solanacearum SL341 | This study |
| pRKR | Tcr; pRK415 carrying 1.22 kb EcoR1 fragment of pGEMR containing oxyR gene of R. solanacearum SL341 | This study |
| pRKG | Tcr; pRK415 carrying 1.16 kb Pst1 fragment of pGEMG containing hrpG gene of R. solanacearum SL341 | This study |
| pRK2013 | Kmr; mobilization helper plasmid for triparental mating | 42 |
Kanr, chromosomal kanamycin resistance; Apr, ampicillin resistance; Tcr, tetracycline resistance; Kmr, kanamycin resistance