Fig 2. Growth properties of purified N27-A and unpurified N27 cells.

(A-F): Both cell types were plated at 20,000 cells in each well of 6-well plates. Representative images were taken at Day 1, 3, and 5 for each cell type. Purified N27-A cells (Images A-C) grew more slowly than unpurified N27 cells (Images D-F). (G): Growth charts of purified N27-A (red line) and unpurified N27 cells (green line) from Day 0 to Day 7. Data present the average cell number from two wells of purified and unpurified cells in three experiments (n = 6, each cell type). Bar, 20 μm for A-F.