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. 2016 Aug 11;11(8):e0160847. doi: 10.1371/journal.pone.0160847

Fig 4. Dopamine neuron transcription factors (Nurr1, En1, FoxA2 and Pitx3) and VMAT2 and DβH in purified N27-A and unpurified N27 cell cultures.

Fig 4

N27 cells were cultured on 8-well chamber slides and immunostained with the dopamine neuron transcription factors Nurr1 (A-D), En1 (E-H), FoxA2 (I-L) and Pitx3 (M-Q), as well as VMAT2 (R-U) and DβH (V-Y). Cells were also stained for the nuclear marker DAPI. (A-B): In N27-A clone, nearly all cells had strong Nurr1 staining as shown by Nurr1 and DAPI double staining. (C-D): In unpurified N27 cells, only a small fraction of cells expressed Nurr1. (E-F): In N27-A cells, most cells had strong En1 staining. (G-H): In unpurified N27 cells, nearly all cells were negative for En1 staining. (I-J): In N27-A clone, cells were moderately stained for FoxA2. (K-L): In unpurified N27 cells, nearly all cells were FoxA2 negative. (M-Q): In N27-A clone, cells were faintly stained for Pitx3. In unpurified N27 cells, no cells expressed Pitx3. (R-U): In N27-A clone, most cells were positive for VMAT2. In unpurified N27 cells, only a few cells were VMAT2-positive. (V-Y): In both N27-A and N27 clones, no cells were DβH-positive. Bar, 50 μm for A-Y.