Fig 6. Purified N27-A cells are more sensitive to 6-OHDA toxicity than unpurified N27 cells.

Purified N27-A and unpurified N27 cells were cultured in 24-well plates (for trypan blue staining) and 96-well plates (for MTT assay). Two days after plating, cells were treated with 0–150 μM of 6-OHDA for 24 hr. The cell viability was measured by trypan blue staining (A) and MTT assay (B). (A): Cell viability data from trypan blue staining showed that there were significantly fewer viable cells in purified N27-A cultures compared to unpurified N27 cultures. (B): Cell viability results from the MTT assay also showed that purified N27-A cells had greater cell death than unpurified N27 cells after exposure to 6-OHDA. Reduced cell survival in both assays indicate that purified N27-A cells are more sensitive to 6-OHDA than unpurified N27 cells (n = 12 for A, n = 15 for B, **p<0.01).