Fig 8. H₂O₂ toxicity is similar in purified and unpurified N27 cells.

Purified N27-A cells and unpurified N27 cells were cultured in 24-well plates (for trypan blue staining) and 96-well plates (for MTT assay). Two days after plating, cells were treated with 0–200 μM of H₂O₂ for 24 hr. Cell viability was measured by trypan blue staining (A) and MTT assay (B). (A): Cell viability data from trypan blue staining showed similar numbers of viable cells in purified and unpurified N27 cells. (B): Cell viability results from the MTT assay revealed no significant differences in surviving cells from purified N27-A or unpurified N27 cells indicating that both cell sources had similar sensitivity to the non-specific toxicity of H₂O₂ (n = 12 for A, n = 15 for B, **p<0.01).