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. 2016 Aug 11;12(8):e1005790. doi: 10.1371/journal.ppat.1005790

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© 2016 Groen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Score scatter plot from principal component (PC) analysis of m/z values (binned to 1.0 Da) obtained by gas chromatography-mass spectrometry of samples of volatile organic compounds (VOCs) collected by dynamic headspace trapping from tomato plants that had been mock-inoculated (blue), infected with CMV (strain Fny) (red) or CMVΔ2b-infected (green). The analysis shows discrimination between all three treatments. The percentage of variation of the data explained by PC1 and PC2 is in parentheses (80.3 and 9.8%, respectively). (B) Whole plant total emission rate (ng.h^-1) of the combined (most abundant) volatiles is similar for mock-inoculated and virus-infected plants. (C) VOC emission rate (ng.h^-1) per gram dry weight of the combined (most abundant) volatiles is highest from CMV-infected plants compared to mock-inoculated and CMVΔ2b-infected plants. (D) Whole plant VOC emission rate (ng.h^-1) for the five most abundant volatiles from mock-inoculated, CMV-infected and CMVΔ2b-infected tomato plants. (E) VOC emission rate (ng.h^-1) per gram dry weight of the five most abundant volatiles from mock-inoculated, CMV-infected and CMVΔ2b-infected tomato plants show that pinene and cymene emission are significantly higher in CMV-infected plants compared to mock-inoculated and CMVΔ2b-infected plants. The mean VOC emission values for combined or individual volatiles are presented (n = 3 plants per treatment). Error bars represent standard error of the mean. The level of significance is shown by a p-value calculated with one-way ANOVA and post hoc Tukey HSD testing.