Fig 7. Unaltered gene expression of urea and TCA cycling, but decreased ATP5B mRNA in liver after thermal stress in early lactation.

mRNA abundances of (A) cytosolic argininosuccinate lyase (ASL), (B) mitochondrial carbamoyl-phosphate synthase (CPS), (C) citrate synthase (CS), (D) NADH dehydrogenase subunit 2 (ND2), (E) mitochondrial F1 complex of ATP synthase, beta polypeptide (ATP5B) in liver of heat-stressed (HS) and or pair-fed (PF) cows. Liver samples were obtained after ad libitum feeding in P1 at thermoneutral condition (P1 (TN-AL), black) and after 6 days (P2) of HS (red) or PF (blue) cows in the ante partum (ap) and post partum (pp) period, respectively. Data are from HSap n = 6, PFap n = 5, HSpp n = 5, PFpp n = 3 RNA samples.* P<0.05.