The levels of VEGF (A) and ERK (B) was significantly increased after IR (IR vs. control, p<0.05). However, the IR-induced expression of VEGF and ERK was attenuated in B5-IR (p<0.05). There was a significant difference from the *CTRL (p<0.05) and #IR (p<0.05) groups. Anti-VEGF antibody, anti-vascular endothelial growth factor antibody; IR, ischemia-reperfusion; ALI, acute lung injury; ERK, extracellular signal-regulated kinase. CTRL, control group; B5-CTRL, control + preconditioning bevacizumab, 5mg/kg group; IR, ischemia-reperfusion group; B1-IR, IR + preconditioning bevacizumab, 1mg/kg group; B5-IR, IR + preconditioning bevacizumab, 5mg/kg group; IR-B5, IR + post-IR bevacizumab, 5mg/kg group.