Fig. 1.

A. Rat aortic SMC and rat BMDM were incubated separately with either 1 μM or 10 μM SPC or vehicle for 24 h in presence (black bars) or absence (white bars) of 10 ng/ml TNF. SMC were subsequently co-cultured with BMDM for 15 min and adherent BMDM counted (n = 8). Scale bar = 250 μm. B. Rat aortic SMC were incubated with 10 μM SPC ± 10 ng/ml TNF for 24 h and BMDM were incubated separately with 10 ng/ml TNF only for 24 h (n = 12). Rat aortic SMC were subsequently co-cultured with BMDM for 15 min and adherent BMDM counted. C. Rat aortic SMC were incubated with 10 ng/ml TNF for 24 h and BMDM were incubated separately with 10 μM SPC ± 10 ng/ml TNF for 24 h. Adherent BMDM were assessed as above (n = 6). Results are presented as fold change of adhesion relative to vehicle treatment. Data are expressed as mean ± sem, ∗ denotes p < 0.05, NS denotes not significant.