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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Neurosci Res. 2015 Nov 3;94(3):207–230. doi: 10.1002/jnr.23685

Figure 10. Intra-axonal dye diffusion and axon morphology in allografts.

Figure 10

From Riley et al., 2015) with permission.

A, B. No intra axonal dye diffusion of Texas Red at 1d postoperatively in a negative control allograft (A) but observed for a PEG-fused allograft (B). Arrow: site of proximal cut end of host sciatic nerve micro-sutured to proximal end of donor sciatic allograft.

C–G. Increased myelinated axon viability for PEG-fused allografts compared to negative controls or unsuccessful PEG-fusion at 6w postoperatively. Toluidine-blue, plastic embedded sections of sciatic nerves viewed at 20× for an un-operated control (C) and the mid-allograft region at 6wk postoperatively for a negative control (D) and PEG-fused sciatic nerves showing excellent (E), good (F) or no (G) behavioral recovery as assessed by SFI scores (see Fig. 11 and Riley et al., 2015). Note that successful PEG-fusion is correlated with survival of increased numbers of larger diameter myelinated axons within the allograft segment.