. Author manuscript; available in PMC: 2017 Aug 27.

Published in final edited form as: Nat Prod Rep. 2016 Jul 4;33(8):942–950. doi: 10.1039/c6np00024j

Table 1.

Pros and cons for the detection methods most used for natural products dereplication

Detection method	Pros	Cons
UV/Vis	• Inexpensive • Non-destructive • Easily placed in-line with MS	• Provides little structural information • Compounds must be UV-active
MS	• Very sensitive (~ng) • Provides molecular formula (MS¹) • Can provide substructures (MSⁿ)	• Ionization is highly dependent on compound and conditions • Can be very expensive • No good spectral databases for MS² • Destructive
NMR	• Highest level of structural detail • Applicable to all compounds • Non-destructive	• Very expensive • Low sensitivity