Table 1. Overview of SELEX procedure.

The procedure consists of four main steps; the numbering for each sub-step is related in the following section.

Main Steps		Sub-Steps	Time
2.1.	Basic preparations	2.1.1. Reagents and material preparations
		2.1.2. Instruments/Equipment
		2.1.3. Design of library, primers and capture
2.2.	Selection procedure	2.2.1. Choosing the concentration of target solution and compounds for negative selection	Iterative step. 1 ~ 1.5 round of SELEX per day. Approximately, ~ 3 weeks
		2.2.2. Selection step & elution profile
		2.2.3. Large scale PCR & strand separation
2.3.	Post-selection step	2.3.1. Cloning & sequencing	4 ~ 7 days
		2.3.2. Sequence analysis
		2.3.3. Clone SELEX
2.4.	Aptameric sensor validation	2.4.1. Fluorescent sensor ordering	4 ~ 7 days
		2.4.2. Analysis of sensor reactivity	> 1 day
		2.4.3. Affinity measurement	~ 1 day
		2.4.4. Further optimization tips	Case by case