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. 2016 Aug 12;6:31572. doi: 10.1038/srep31572

Figure 2. Morphine decreases co-culture-induced IL-6, TNF-α and VEGF-A production.

Figure 2

Conditioned media of 4T1 and RAW264.7 cell co-cultured in the presence (20 μM) or absence of morphine were added to mouse angiogenesis antibody array membranes. (a) The images were captured using a ChemiDoc Touch Imaging System (Bio-Rad Laboratories Inc.) Green, Blue and red circling were added to locate duplicate spots corresponding to VEGF, TNF-α and IL-6, respectively. (b) Results are normalized to positive control signal intensities and angiogenic factors expressed as % of the amount measured in the no-morphine control. Graph shows mean ± S.D. (N = 3 separate experiments, each performed with a mix of three independent co-culture media). *P = 0.05.