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. 2016 Aug 12;6:31572. doi: 10.1038/srep31572

Figure 3. Effect of morphine on the production of pro-angiogenic factors IL-6, TNF-α and VEGF-A.

Figure 3

Protein level of (a) IL-6, (b) TNF-α and (c) VEGF–A in the conditioned media of 4T1, RAW264.7 alone or in co-culture in the presence (10 μM and 20 μM) or absence of morphine was measured by ELISA. (d) ELISA quantification of VEGF-A present in the conditioned medium of RAW264.7 cells co-cultured with 4T1 in the absence or presence of morphine at indicated concentrations (10 nM, 100 nM, 500 nM, 1 μM, 5 μM, 10 μM and 20 μM). Results are shown as the mean of growth factor concentrations ± S.D. in three independent experiments (N = 3), *P = 0.05.