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. 2016 Aug 12;6:31578. doi: 10.1038/srep31578

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative electron micrographs from TEM of Spurr resin-sectioned (100 nm sections) native THP-1 cells (a,e,i), THP-1 cells co-cultured with 50 μg/mL Alhydrogel^® (24 h) (b,f,j) and 50 μg/mL Adju-Phos^® (Brenntag Biosector, Denmark) adjuvant (24 h) (c,g,k) and 50 μg/mL Imject™ Alum (Pierce, Thermo Scientific) adjuvant (24 h) (d,h,l). Cell resin-sections were stained for 20 min with 2% ethanolic uranyl acetate, rinsed with 30% ethanol followed by ultrapure water and finally allowed 24 h drying time prior to analysis via TEM. Inserts show close-ups of intracellular adjuvant particles contained within vesicle-like structures and the red arrows highlight their presence within the respective cell images. Magnification and scale bars: (a–c) X 8 K, 5 μm, (d) X 10 K, 2 μm, (e) X 15 K, 2 μm, (f–h) X 30 K, 1 μm, (i) X 30 K, 1 μm and (j–l) X 60 K, 0.5 μm, respectively.