Figure 3. Gross phenotype of ift-139 mutant.

(a–c) A single worm was placed on OP50 feeder bacteria and incubated overnight. (a,b) Representative image of wild type (a) and ift-139(b). (c) Relative moving area was calculated. Mean ± standard deviation (S.D.). *p < 0.01, t-test, n = 10. Note that ift-139(gk477) mutants move in a narrow area compared with wild type. Bars, 1 cm. (d) Gross phenotypes in ift-139 mutants were compared with wild type, osm-3, and dyf-5 mutants. daf, dauer formation, dyf, dye filling and mating: male mating. wt means the phenotype was comparable to wt, - means the defects in the phenotype. (e) Chemotaxis to diacetyl was observed and chemotaxis index was calculated as described in the materials and methods. Mean ± S.D. n = 10 experiments. *p < 0.01, t-test.