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. 2016 Aug 12;6:30950. doi: 10.1038/srep30950

Figure 3. Reproduction of experimental data dedicated for parameter estimation using model with sensitized negative feedback.

Figure 3

Relative Hog1 and Slt2 phosphorylation data and relative single cell volume measurements, used for models parameters estimation, are plotted versus time. Simulations were done using the best ranked model from the ensemble of models with sensitized negative feedback. Solid lines show model simulations and filled circles (•) show the experimental data (Mean ± SD (n = 3)). (a) Comparison between Hog1 phosphorylation data and respective simulation for 0.8 M sorbitol shock only (NoHYPOS-Exp) and 4′, 14′ and 30′ hypo-shock experiments using the best ranked model (4 minHYPOS, 14 minHYPOS, 30 minHYPOS, respectively). (b) Comparison between Slt2 phosphorylation data and its simulation for 0.8 M sorbitol shock only, 4′, 14′, 30′ hypo-shock using best ranked model. The selected model can reproduce the 4′ Slt2 activation. (c) Comparison between the relative value of single cell volume measurement and its simulation upon 0.8 M sorbitol shock. The same color code was used for panel a,b.