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. 2015 Nov 17;34(2):418–430. doi: 10.1002/stem.2241

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© 2016 The Authors STEM CELLS published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Osteogenic differentiation in bone marrow mononuclear cells (BMMNCs) and STRO‐1‐selected populations is promoted by early transient Wnt exposure but is abrogated by prolonged Wnt stimulation. (A): Transient, early Wnt stimulation causes a significant increase in ALP activity in comparison with control in basal and osteogenic media in general bone marrow cell populations as well as STRO‐1‐selected cells while prolonged Wnt stimulation has the opposite effect (B). This effect is reflected in Alizarin Red staining in cells cultured in osteogenic media (C) or by absorbance measurement on the extracted dye (D). Gene expression analysis shows a trend towards an increase in RUNX2 and BGLAP expression after short‐term Wnt induction and decrease after long‐term Wnt induction (E). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. Abbreviations: BMMNCs, bone marrow mononuclear cells; PNPP, p‐nitrophenyl phosphate; REU, relative expression units.