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. 2016 Aug 11;16:176. doi: 10.1186/s12870-016-0863-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Main stages of the two microspore pathways: gametophytic development and microspore embryogenesis. a-g Semithin sections, toluidine blue staining. a Vacuolated microspore. b-c Gametophytic development, in vivo. b Bicellular pollen. c Tricellular mature pollen. d-h Microspore embryogenesis, in vitro. d Two-cell proembryo. e Multicellular proembryo. f Globular embryo. g Late torpedo embryo. h Panoramic view of cotyledonary embryos in the Petri dish. Ex: exine, V: vacuole. Bars: a-d, 10 μm; e, f, 20 μm; g, 50 μm