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. 2016 Aug 11;16:176. doi: 10.1186/s12870-016-0863-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression analysis of BnPME gene by RT-PCR during gametophytic development and microspore embryogenesis. Semiquantitative RT-PCR representative agarose electrophoresis gels of the amplification products. Histogram expresses relative changes in the PME bands intensities at different developmental stages: vacuolated microspore, differentiated pollen (gametophytic development), early proembryo, globular embryo, cotyledonary embryo (microspore embryogenesis). Columns represent means of mRNA expression of BnPME ± SE (bars) of three independent experiments with three replicates each, in arbitrary units (a.u.). Asterisks indicate significant differences with the vacuolated microspore (Student’s t-test at P ≤ 0.05)