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. 2016 Aug 11;16:176. doi: 10.1186/s12870-016-0863-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Immunolocalization of esterified and non-esterified pectins during gametophytic development. a, b, c are the same structures, visualized by DIC (Differential Interference Contrast, Nomarsky) as in a’, b’, c’. a’, b’, c’ Immunofluorescence of esterified (JIM7 antibody) and non-esterified (JIM5 antibody) pectins during gametophytic development. a, a’, d Vacuolated microspore; b, b’, e Bicellular pollen; c, c’, f Tricellular mature pollen. a’, b’, c’ Merged images of fluorescence provided by DAPI staining of nuclei (in blue) and JIM5 immunofluorescence signal (in green). d, f Merged images of fluorescence provided by DAPI staining of nuclei (in blue) and JIM7 immunofluorescence signal (in green). e Control immunofluorescence experiment avoiding the first antibody. Ex: exine, N: nucleus, V: vacuole. Bars: 10 μm