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. 2016 Aug 11;16:176. doi: 10.1186/s12870-016-0863-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Immunolocalization of esterified and non-esterified pectins during microspore embryogenesis. Confocal merged images of immunofluorescence signal (green) and DAPI staining of nuclei (blue). For some stages, DIC image (Differential Interference Contrast, Nomarsky) of the same section is shown to reveal the structure (left side for each pair of images). a, a’, c, c’, e, g JIM7 immunofluorescence. b, b’, d, d’, f, h) JIM5 immunofluorescence. a-d Two-cell and early proembryos. e, f Globular embryos. g, h Cotyledonary embryos. Bars: a-d) 10 μm; e-h) 25 μm