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. 2016 Aug 11;15:139. doi: 10.1186/s12934-016-0540-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Proof-of-principle of in vivo recombination (two-fragment assembly). a Schematic diagram of fragments used for testing in vivo recombination using pARS-BFP as template. b Frequencies of transformation obtained using overlaps of 100, 50, or 20 nucleotides between fragment 1 and fragments 2, 3, or 4. c BFP fluorescence signal obtained from 8 independent colonies randomly selected from the different pools (ns non-significant, according to Sidak’s multiple comparison test on one-way ANOVA, α 0.05)