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. 2016 Apr 4;211(3):940–951. doi: 10.1111/nph.13938

Figure 6.

Figure 6

Abscisic acid (ABA) insensitivity of the Arabidopsis thaliana arabidillo mutant during seed germination and its rescue by both PHYSCODILLO and SELAGIDILLO proteins. (a) Seeds from wild‐type (Col‐0), arabidillo mutant, a 35S::ARABIDILLO‐YFP overexpressing line (Coates et al., 2006) and an arabidillo mutant stably expressing pARABIDILLO1::ARABIDILLO1‐YFP (Coates et al., 2006; Nibau et al., 2011) plated on 1 μM ABA with percentage germination assayed over 7 d. Error bars show ± SE of the mean. A Kruskal–Wallis test identified significant (P < 0.05) differences between genotypes on days 2, 3, 4 and 5. Different letters indicate significant (P < 0.05) differences between genotypes determined using Dunn's test for, top to bottom, arabidillo1/2, wild‐type, 35S::ARABIDILLO1‐YFP in wild‐type, and pARABIDILLO1::ARABIDILLO1‐YFP in arabidillo1/2. (b) Seeds from wild‐type, arabidillo mutant, and arabidillo mutant stably expressing PHYSCODILLO1 (35S::PHYSCODILLO1‐GFP; two independent lines, Rescue 1 and Rescue 2) plated on 1 μM ABA with percentage germination assayed over 7 d. A Kruskal–Wallis test identified significant differences (P < 0.05) between genotypes on days 2, 3, 4, 5 and 6. Different letters indicate significant differences between genotypes determined using Dunn's test (P < 0.05) for data points, from top to bottom, for arabidillo1/2, wild‐type, Rescue 1 and Rescue 2. (c) Seeds from wild‐type, arabidillo mutant, and arabidillo mutant stably expressing SELAGIDILLO (35S::SELAGIDILLO‐GFP: two independent lines, Rescue 1 and Rescue 2) plated on 1 μM ABA with percentage germination assayed over 7 d. A Kruskal–Wallis test identified significant differences (P < 0.05) between genotypes on days 2, 3, not 4 (P = 0.07), 5, 6 and 7. Different letters indicate significant differences between genotypes determined using Dunn's test (P < 0.05) for data points, from top to bottom, for arabidillo1/2, wild‐type, Rescue 1 and Rescue 2.