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. 2016 Mar 17;283(8):1531–1549. doi: 10.1111/febs.13690

Figure 5.

Figure 5

Binding of AF, RoF, SAM and SAH to RosA. Calorimetric titrations of RosA with AF (A), RoF (B), SAM (C) and SAH (D) were performed in 50 mm Tris/HCl buffer, pH 8.0, containing 100 mm NaCl at 25 °C using a VP‐ITC system (MicroCal). The dissociation constants for binding of AF, RoF, SAM and SAH to RosA were 10 ± 1, 0.8 ± 0.05, 22 ± 2 and 2 ± 0.1 μm, respectively (three independent measurements for each ligand). Data were analysed by non‐linear least‐squares fitting using origin version 7.0 (MicroCal).