Table 4.
Primer pairs used for molecular characterization of Fusarium isolates with product size, annealing temperature and relevant publications.
| Gene* | Primers | Sequence 5′–3′ (forward primer/reverse primer) | Product size | Annealing temperature (ºC) | Publication |
|---|---|---|---|---|---|
| TUB2 | T1/T22 | AACATGCGTGAGATTGTAAGT/TCTGGATGTTGTTGGGAATCC | ∼1500 | 60 | O'Donnell and Cigelnik (1997) |
| RPB2 | 7cF/11aR | ATGGGYAARCAAGCYATGGG/GCRTGGATCTTRTCRTCSACC | 881 | 57 | O'Donnell et al. (2007) |
| EF‐1α | exTEF‐F/FUexTEF‐R | ACCCGGTTCAAGCATCCGATCTGCGA/AGCTTGCCRGACTTGATCTCACGCTC | 1269 | 64 | Vágány (2012) |
| SIX1 | SIX1 | GTATCCCTCCGGATTTTGAGC/AATAGAGCCTGCAAAGCATG | 992 | 59 | Lievens et al. (2009) |
| SIX2 | SIX2 | CAACGCCGTTTGAATAAGCA/TCTATCCGCTTTCTTCTCTC | 749 | 59 | Lievens et al. (2009) |
| SIX3 | SIX3 | CCAGCCAGAAGGCCAGTTT/GGCAATTAACCACTCTGCC | 608 | 59 | Lievens et al. (2009) |
| SIX4 | SIX4 | TCAGGCTTCACTTAGCATAC/GCCGACCGAAAAACCCTAA | 967 | 59 | Lievens et al. (2009) |
| SIX5 | SIX5 | ACACGCTCTACTACTCTTCA/GAAAACCTCAACGCGGCAAA | 667 | 59 | Lievens et al. (2009) |
| SIX6 | SIX6 | CTCTCCTGAACCATCAACTT/CAAGACCAGGTGTAGGCATT | 793 | 59 | Lievens et al. (2009) |
| SIX7 | SIX7 | CATCTTTTCGCCGACTTGGT/CTTAGCACCCTTGAGTAACT | 862 | 59 | Lievens et al. (2009) |
| SIX8 | SIX8 | TCGCCTGCATAACAGGTGCCG/TTGTGTAGAAACTGGACAGTCGATGC | 250 | 59 | Meldrum et al. (2012) |
| SIX9 | FOL SIX9 | GGGTGGACCATATCACGATGTTCG/GAATACCTGAGTGGAGTTGTGTCTTG | 458 | 69 | This study |
| SIX9 | FOC SIX9 | GGCCCAGCCCTAGTCTAACTCC/AACTTAACATGCTGGCCGTCAATCG | 347 | 67 | This study |
| SIX10 | SIX10 | GTTAGCAACTGCGAGACACTAGAA/AGCAACTTCCTTCCTCTTACTAGC | 636 | 65 | This study |
| SIX11 | SIX11 | ATTCCGGCTTCGGGTCTCGTTTAC/GAGAGCCTTTTTGGTTGATTGTAT | 559 | 61 | This study |
| SIX12 | SIX12 | CTAACGAAGTGAAAAGAAGTCCTC/GCCTCGCTGGCAAGTATTTGTT | 449 | 61 | This study |
| SIX13 | SIX13 | CCTTCATCATCGACAGTACAACG/ATCAAACCCGTAACTCAGCTCC | 1027 | 61 | This study |
| SIX14 | FOL SIX14 | ATAAAGTGCGACTGGACTTCTGCC/ACCCCCATCCACATTCCTAAGCGA | 422 | 67 | This study |
| SIX14 | FOL SIX14 nest | GATCCCAATGGGGGCTGTGT/GCTGGTGGCTAGAATCTCTTTGGA | 232 | 59 | This study |
| SIX14 | FOC SIX14 | ACAACACCGCGACGCTAAAAAT/GCACACTCAGTGCGACAAGTTC | 438 | 61 | This study |
| C5 | C5 | AGAGTGTGAAGTGAGGACGAGGGA/CTACGTTCGCCTCACTCATTGCCT | 1064 | 63 | This study |
| CRX1 | CRX1 | CACCATCTGTCTACATAAGGCCGCCC/AAAGTTCAAGGACCGGACCGCCG | 1654 | 69 | This study |
| CRX2 | CRX2 | TTAGTCGCACATCTACCATCACTG/GGAGTCGATCTAACTTCAGG | 856 | 58 | This study |
| CRX2 | CRX2 FP | CCAGTGCATTGGTTTGAGACGTT/ATGCGCTCGCTTTCTATGTATCTG | 902 | 63 | This study |
| Primers used for real‐time reverse transcription‐polymerase chain reaction (RT‐PCR) | |||||
| SIX3 | QSIX3 | GGCCGTCTTCTACTTCATTTAC/GGGAGAATGTTCTAGCATAACC | 69 | 63 | This study |
| SIX5 | QSIX5 | TGGGCTCGAAAAGTCCAGCAT/TGTTTCGCCGTCAATGTCGCC | 114 | 63 | This study |
| SIX7 | QSIX7 | TCGATCTCTTTCCAAGACAAGGGCA/GTGGACGCGGCGTTGGTGAAC | 130 | 63 | This study |
| SIX9 | QSIX9 | GCCGACCCAGACCTACGCTTT/GCTGGTTTTGGAAGCCCAGTTGT | 129 | 63 | This study |
| SIX10 | QSIX10 | CCCGGAAAGCCTGCATCGACTA/AGAACAAACGTCGGTGGGACCA | 53 | 63 | This study |
| SIX12 | QSIX12 | TGCTGCTCCAAGTACAAACTACCTT/GCTGATACCTTTGGGTCCAACGC | 71 | 63 | This study |
| SIX14 | QSIX14 | ATGTCGTATGCCGGACGGGAA/TTATCTCGTAGACGCCTTCCT | 109 | 60 | This study |
| C5 | QC5 | GCCTATGGCAGGACTTGTTGAC/CCACAGCTTCTTGGACTATCTCC | 126 | 63 | This study |
| CRX1 | QCRX1 | AACTCAGGTACCACATCGGGA/CAGGTCGTCCTAGCGTCAGT | 89 | 60 | This study |
| CRX2 | QCRX2 | CAATCAGAAACCACGACGGAA/GGAGTCGATCTAACTTCAGG | 89 | 60 | This study |
| EF‐1α | QTEF | GGTCAGGTCGGTGCTGGTTACG/TGGATCTCGGCGAACTTGCAGG | 77 | 63 | This study |
| TUB2 | QTUB | TTCTGCTGTCATGTCCGGTGT/TCAGAGGAGCAAAGCCAACCA | 134 | 63 | This study |
*EF‐1α, translation elongation factor 1α; RPB2, RNA polymerase II second largest subunit ; SIX, Secreted In Xylem; TUB2, β‐tubulin.