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. 2016 Feb 15;18(4):1264–1276. doi: 10.1111/1462-2920.13203

Figure 5.

figure

Physical characterization of gas vesicles and volumetric calculations in E . coli and S39006. (A) Collapse pressure experiments on S39006 or (B) E . coli carrying the pGAS cosmid. At 0.05 MPa pressure increments, the relative proportion of gas vesicles present in each sample was ascertained. The values are the average of three biological replicates ± SD. The black dashed lines indicate the mean collapse pressure, p c, for each sample and the continuous black line indicates p t, the turgor pressure. Where indicated, the collapse pressure was measured in cells exposed to LB medium containing 0.35 M sucrose. (C) Volumetric calculation of the percentage of gas vesicles in cells. Values indicated are the mean gas vesicle percentage of three biological replicates ± SD.