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. 2015 Oct 21;45(12):3431–3440. doi: 10.1002/eji.201546018

Figure 5.

Figure 5

CD25 expression is induced by P. yoelii iRBC and is absolutely dependent upon IL‐18. (A–D) Splenocytes from WT C57BL/6 (B6; black bars), IL‐12p35−/– (gray bars), and IL‐18R−/− (white bars) mice were cultured for 19 h (with Brefeldin A present for the last 3 h) with uRBCs and iRBCs in the presence or absence of blocking antibodies to IL‐12 and IL‐18 or isotype control IgG antibody, and analyzed for (A, B) CD25 and (C, D) IFN‐γ expression. The entire dataset from one of two experiments is shown in (A) and (C) and statistical analyses for iRBC‐stimulated cells are shown in (B) and (D) (n = 3). ANOVA with Tukey's multiple comparison test was applied. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.