Figure 4.

D-JNKI inhibits LPS-induced cPLA2 phosphorylation and production of COX-2, CCL-2 and CXCL-1 in myometrium. Timed pregnant CD-1 mice were injected on day 16 with 1.0 μg of Salmonella abortus LPS alone, LPS together with 5 μg of D-JNKI or with 5 μg of D-JNKI alone. Tissue samples from the myometrium were collected at 6 h and analysed for mRNA and protein levels of COX-2 (A and D), CCL-2 (B and E) and CXCL-1 (C and F) expression and normalised to loading controls (GAPDH and actin) as arbitrary units (AU). Data represent the mean (±s.e.m.) of three animals at each data point. The difference between LPS alone or together with D-JNKI was statistically significant by ANOVA *P<0.05 and **P<0.01.