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. 2016 Aug 11;17:614. doi: 10.1186/s12864-016-2967-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — RT-qPCR validation of time-course RNA-seq data using six selected lincRNAs differentially expressed over time. 18S rRNA and elongation factor 1-α (ef1α) were used as the reference genes. The relative expression levels of lincRNAs at each time point were calculated relative to calibrator (control sample; 0 hpi). Error bars represent the range of relative expression (fold change) calculated by 2^{-(ΔΔCt±SD)}. Two biological replicates were used in triplicate