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. 2016 Aug;22(8):1464–1467. doi: 10.3201/eid2208.150696

Table. Primer sequences of microsatellite loci used in multilocus sequence analysis of Cyclospora cayetanensis.

Locus Contig no. Targeted repeat* Primer sequence, 5′ → 3′† Annealing temp, °C Expected size, bp Amplification efficiency, no. positive/no. analyzed
CYC3 00003 TGTA63 and TATA23 F1: GAAGATGAAGCGTTGGTACG; 
R1: TACCGCTGCTGGAGTGCAT; 
F2: TTGTGCATGGCACCCAATGC; 
R2: CCAGACAGTAGTTCGTGTCTT 55 598 4/4
CYC13 00008 GAT15 F1: TTGGAGCAGGACGAGTTTCG; 
R1: ATGGAAGCGGCTATGAAATTGG; 
F2: CCTCGGAGTCCTCTGAGTG; 
R2: AGCCGTCGCAGTGTGTAGCA 58 595 4/4
CYC15 00009 TGC11 F1: AGTAGCTACGTGCCAAGACGA; 
R1: TCGTTCTATCTGACCATAGTAGTG; 
F2: CGCTGTGCAAGAGGCGATCTA; 
R2: AAGCACTGCAGGGTCCGTAAC 58 609 4/4
CYC21 00036 AT31 F1: TAGTGGCGACTGCGACATG; 
R1: GCACCTTGCTGATGAGGCA; 
F2: CTA AGGCTGTCTTGAGCGG; 
R2: CGCCCACATGCTTCGTATAC 55 471 4/4
CYC22 00037 AC20 F1: CACTATGCCGTGTGACACGT; 
R1: GTAGATTTGCAAGAACTCATGCTA;
F2: ATAGTATTCAGGCGCAAACTAAG; 
R2: GAGGCTTTCCAAAGGTCTAGTT 55 512 4/4

*Tandem repeat identified in the sequence from whole genome sequencing.
†Six C. cayetanensis specimens were used in initial evaluation of PCR primers: specimens 22231, 22234, 22238, and 28709 were used to evaluate PCR primers from loci CYC3, whereas specimens 22231, 22234, 24550, and 24552 were used for PCR primers from the remaining loci.