Table. Results of testing HCF from 21 patients whose samples had tested positive for Orientia tsutsugamushi by qPCR on EDTA buffy coat after >1 days of incubation, Laos, 2014* .
| Patient code |
Day of incubation of HCF† | ||||
|---|---|---|---|---|---|
| 1 |
2 |
3 |
5 |
7 |
|
| 29931 | + (Cq ≈34) | ||||
| 30009 | – | ||||
| 30029 | – | ||||
| 30100 | – (Cq ≈42) | – | + (Cq ≈35) | + (Cq ≈38) | |
| 30104 | + (Cq ≈38) | ||||
| 30316 | + (Cq ≈37) | – | + (Cq ≈38) | ||
| 30329 | + (Cq≈39) | + (Cq ≈35) | + (Cq ≈37) | ||
| 30379 | + (Cq ≈34) | ||||
| 30416 | + (Cq ≈35) | + (Cq≈40) | + (Cq ≈40) | ||
| 30442 | – | – | |||
| 30446 | + (Cq ≈37) | – | |||
| 30581 | – | + (Cq≈32) | + (Cq ≈30) | + (Cq ≈34) | |
| 30600 | – | + (Cq ≈32) | |||
| 30862 | + (Cq ≈33) | ||||
| 30874 | + (Cq ≈30) | ||||
| 30920 | |||||
| 31120 | + (Cq ≈31) | ||||
| 31199 | + (Cq ≈30) | ||||
| 31209 | + (Cq ≈31) | + (Cq ≈21) | |||
| 31230 | + (Cq ≈31) | + (Cq ≈31) | + (Cq ≈32) | ||
| 31231 | + (Cq ≈32) | + (Cq ≈30) | + (Cq ≈37) | ||
*For each patient, 2 hemocultures bottles were used (Pharmaceutical Factory No. 2, Vientiane, Laos): 1.7 g tryptic hydrolysate casein, 0.3 g soy peptone, 0.5 g sodium chloride, 0.25 g potassium phosphate, 0.25 g dextrose, 0.025 g sodium polyanetholsulfonate/100 mL) were inoculated with blood in a 1:10 dilution. Cq, cycle quantitation; HCF, hemoculture fluids; qPCR, quantitative PCR; –, sample available/negative; +, sample available/positive; blank cell, no sample available. †Cq values are noted in parentheses to illustrate the increase in template.