Table 1.
Results of the molecular analysis and screening of the bioactive potential of the studied Planctomycetes.
| Strain | Genera | Bioactivity against Candida albicans | Bioactivity against Bacillus subtilis | PKS-I size amplicon | NRPS size amplicon | PKS-I genes closest similar result using blastn in NCBI | Similarity; Coverage | PKS-I genes closest similar result using blastp in NCBI | Similarity; Coverage | NaPDoS prediction pathway product | NRPS genes closest similar result using blastn in NCBI | Similarity; Coverage | NRPS genes closest similar result using blastp in NCBI | Similarity; Coverage | NaPDoS prediction pathway product |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Rb SH1 | Rhodopirellula baltica | Bioactive | Bioactive | 750 bp | 1000 bp | Rhodopirellula baltica SH 1 complete genome; segment 22/24 | 99%; 99% | polyketide synthase [Rhodopirellula baltica] | 100%; 97% | epothilone | NSR | – | – | – | – |
| UC21 | Rhodopirellula baltica | Bioactive | Bioactive | 750 bp | – | – | – | – | – | – | – | – | – | – | – |
| UC49.1 | Rhodopirellula baltica | Bioactive | Bioactive | 750 bp | 1000 bp | Rhodopirellula baltica SH 1 complete genome; segment 22/24 | 97%; 100% | polyketide synthase [Rhodopirellula baltica] | 100%; 97% | epothilone | – | – | – | – | – |
| FC9.2 | Rhodopirellula sp. | Bioactive | Bioactive | – | 750 bp | Alpha proteobacterium F16 beta ketosynthase gene, partial cds | 85%; 98% | beta ketosynthase, partial [alpha proteobacterium F16] | 88%; 100% | microcystin | Matched with PKSI | – | – | – | – |
| FF4 | Rhodopirellula sp. | Not Bioactive | Bioactive | 1000 bp | 750 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| FC3 | Rhodopirellula rubra | Bioactive | Not Bioactive | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| FC17 | Rhodopirellula rubra | Not assayed | Not assayed | – | 1000 bp | – | – | – | – | – | NSR | – | – | – | – |
| FC15 | Rhodopirellula rubra | Not assayed | Not assayed | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| MsF5.1 | Rhodopirellula rubra | Not Bioactive | Not Bioactive | 750 bp | – | – | – | NSR | – | – | – | – | – | – | – |
| OJF1 | Rhodopirellula rubra | Not assayed | Not assayed | 750 bp | 1000 bp | Rhodopirellula baltica SH 1 complete genome; segment 3/24, dipeptidyl peptidase IV | 90%; 100% | – | – | – | Myxococcus stipitatus DSM 14675, complete genome, non-ribosomal peptide synthetase | 83%; 34% | surfactin synthetase [Rhodopirellula sp. SWK7] | 94%; 98% | HC-Toxin |
| LF2 | Rhodopirellula rubra | Not Bioactive | Not Bioactive | 750 bp | – | Rhodopirellula baltica SH 1 complete genome; segment 11/24 | 73%; 68% | putative membrane protein [Rhodopirellula sp. SWK7] | 97%; 100% | – | – | – | – | – | – |
| UC9 | Rhodopirellula rubra | Not Bioactive | Bioactive | 750 bp | 750 bp | – | – | NSR | – | – | Planctomyces brasiliensis DSM 5305, complete genome, prolyl oligopeptidase | 68%; 40% | Prolyl endopeptidase [Rhodopirellula sallentiNSR SM41] | 98%; 100% | Bacitracin |
| CcC6 | Rhodopirellula lusitana | Not Bioactive | Bioactive | 600 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| CcC8 | Rhodopirellula lusitana | Not Bioactive | Bioactive | 600 bp | 1000 bp | Alpha proteobacterium F16 beta ketosynthase gene, partial cds | 85%; 100% | beta ketosynthase, partial [alpha proteobacterium F16] | 88%; 89% | pikromycin | Matched with PKSI | – | – | – | – |
| FC24 | Rhodopirellula lusitana | Bioactive | Not Bioactive | 750 bp | 1000 bp | Pirellula staleyi DSM 6068, complete genome | 66%; 94% | DUF1501 domain-containing protein [Rhodopirellula sallentiNSR] | 91%; 100% | – | NSR | – | – | – | – |
| FC25 | Rhodopirellula lusitana | Not Bioactive | Bioactive | 750 bp | – | Pirellula staleyi DSM 6068, complete genome | 66%; 93% | DUF1501 domain-containing protein [Rhodopirellula sallentiNSR] | 91%; 100% | – | – | – | – | – | – |
| FC26 | Rhodopirellula lusitana | Not Bioactive | Not Bioactive | 750 bp | – | – | – | NSR | – | – | – | – | – | – | – |
| FC27 | Rhodopirellula lusitana | Not Bioactive | Bioactive | 750 bp | – | – | – | NSR | – | – | – | – | – | – | – |
| SM4 | Rhodopirellula lusitana | Bioactive | Bioactive | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| UC13 | Rhodopirellula lusitana | Bioactive | Bioactive | 750 bp | 1000 bp | – | – | NSR | – | – | Nocardia cyriacigeorgica GUH-2 chromosome complete genome, putative non-ribosomal peptide synthetase (modular protein) | 72%; 60% | hypothetical protein SMAC_05551 [Sordaria macrospora k-hell] | 87%; 82% | – |
| UC16 | Rhodopirellula lusitana | Not Bioactive | Bioactive | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| UC17 | Rhodopirellula lusitana | Bioactive | Bioactive | – | – | – | – | – | – | – | – | – | – | – | – |
| UC20 | Rhodopirellula lusitana | Not Bioactive | Bioactive | – | – | – | – | – | – | – | – | – | – | – | – |
| UC22 | Rhodopirellula lusitana | Not Bioactive | Not Bioactive | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| UC31 | Rhodopirellula lusitana | Bioactive | Bioactive | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| UC33 | Rhodopirellula lusitana | Not assayed | Not assayed | 750 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| UC36 | Rhodopirellula lusitana | Bioactive | Bioactive | 600 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| UC38 | Rhodopirellula lusitana | Not Bioactive | Not Bioactive | 600 bp | 1000 bp | Alpha proteobacterium F16 beta ketosynthase gene, partial cds | 86%; 100% | beta ketosynthase, partial [alpha proteobacterium F16] | 92%; 100% | stigmatellin | Matched with PKSI | – | – | – | – |
| UC49.2 | Rhodopirellula lusitana | Not Bioactive | Not Bioactive | 600 bp | – | – | – | NSR | – | – | – | – | – | – | – |
| UF6 | Rhodopirellula lusitana | Not Bioactive | Bioactive | 1000 bp | 1000 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| LF1 | Rubripirellula obstinata | Not assayed | Not assayed | 750 bp | – | – | – | NSR | – | – | – | – | – | – | – |
| UC8 | Roseimaritima ulvae | Not Bioactive | Not Bioactive | 750 bp | 1000 bp | Chondromyces crocatus strain Cm c5, complete genome, polyketides synthase | 70%; 91% | – | – | myxothiazol | – | – | – | – | – |
| UF2 | Roseimaritima ulvae | Bioactive | Not Bioactive | 1000 bp | – | Brassica rapa subsp. pekinensis clone KBrB081M20, complete sequence | 81%; 16% | – | – | – | – | – | – | – | – |
| UF3 | Roseimaritima ulvae | Bioactive | Not Bioactive | 750 bp | – | Myxococcus hansupus strain mixupus, complete genome, Malonyl CoA-acyl carrier protein transacylase | 66%; 94% | polyketide synthase ketosynthase domain [Nostoc sp. ATCC 53789] | 65%; 99% | stigmatellin | – | – | – | – | – |
| UF4.2 | Roseimaritima ulvae | Bioactive | Not Bioactive | 750 bp | – | Nonomuraea spiralis strain IMC A-0156 pyralomicin biosynthetic gene cluster, complete sequence | 71%; 59% | – | – | stigmatellin | – | – | – | – | – |
| FC18 | New genus | Not Bioactive | Not Bioactive | – | 1000 bp | – | – | – | – | – | – | – | – | – | – |
| FF15 | New genus | Not Bioactive | Not Bioactive | 750 bp | 850 bp | Lyngbya majuscula CCAP 1446/4 clone 7 polyketide sythase gene, partial cds | 83%; 100% | polyketide sythase, partial [Lyngbya majuscula CCAP 1446/4] | 96%; 98% | stigmatellin | NSR | – | – | – | – |
| Pd1 | Planctomyces sp. | Not Bioactive | Not Bioactive | 600 bp | – | – | – | NSR | – | – | – | – | – | – | – |
| UiF1 Ent1 | Planctomyces sp. | Not Bioactive | Not Bioactive | 600 bp | 850 bp | – | – | NSR | – | – | NSR | – | – | – | – |
| Gr7 | Planctomyces brasiliensis | Bioactive | Bioactive | 750 bp | – | Prorocentrum micans polyketide synthase ketosynthase domain protein gene, partial cds | 82%; 99% | polyketide synthase ketosynthase domain protein [Prorocentrum micans] | 98%; 96% | myxalamid | – | – | – | – | – |
NSR, No Sequence Resulted; Blastn was searched in NCBI using somewhat similar sequences and the selected hit was the one with the highest coverage. Blastp was searched in NCBI using somewhat similar sequences and the selected hit was the one with the highest similarity. NaPDoS is a webserver tool that allows the prediction of biosynthetic pathways.