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. 2016 Aug 12;17:625. doi: 10.1186/s12864-016-2990-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Confirmation of microarray results by quantitative real-time PCR. The chosen genes identified by microarray analysis as differentially expressed in palisade parenchyma + upper epidermis (EPP) and spongy parenchyma + lower epidermis (ESP) from leaves of 17-day-old AtHMA4-expressing plants (line 4) and in the wild-type (WT) grown in the presence of 5 μM Zn for one week. Additionally, the expression of LeChln was analyzed. Normalized fold gene expression for a, e metal transporters and uptake facilitators as well as nicotianamine biosynthesis genes; b, f transcription factors; c, g cell wall modification; d, h ethylene pathway genes. Values correspond to means ± SD (n = 3); those significantly different from the WT (Student’s t-test) are indicated by an asterisk (P ≤ 0.05)