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. 2016 Aug 12;17:625. doi: 10.1186/s12864-016-2990-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Zn distribution at cross sections through leaves visualized by Zinpyr-1. Cross sections made through leaves of 17-day-old wild-type (a, c) and AtHMA4-expressing tomato plants from line 4 (b, d) grown for one week in the presence of 0.5 μM Zn – control conditions (a, b), and 5 μM Zn (c, d). Confocal laser scanning microscope (CLSM) settings were different for sections made from leaves grown under control conditions and followed by 5 μM Zn due to the stronger fluorescence of leaves exposed to high Zn. Magnification bars are indicated in the figure