Table 2.

Primers used for plasmid construction

Amplified segment	Oligonucleotide sequences
tasA-sipW-tasA operon(1)	Fwd: 5′- ATGCGGATCCTCAGAGTTAAATGGTATTGCT-3′ Rev: 5′-GCATTCTAGAATTTTTATCCTCGCTATGCGC
tasA-sipW-tasA operon(2)	Fwd.: 5′-ATGCCTCGAGGGATCCTCAGAGTTAAATGGTATTGCT-3′ Rev.: 5′-GCATGCGGCCGCATTTTTATCCTCGCTATGCGC-3′
mCherry	Fwd.: 5′-GATCTCTAGA ATGGTGAGCAAGGGCGAGGAG-3′ Rev.: 5′-GATCGAGCTCGGATCC TTACTTGTACAGCTCGTCCAT-3′
(102-207)EgTrp	Fwd.: 5′ ATGCGCGGCCGCCGAAACATCTACTAAGCTTGAC-3′ Rev.: 5′ GATCCCCCGGGGGATCC TTACTCTTGCTCGGAGACTTCGAG-3′
(102-278)EgTrp	Fwd.: 5′ ATGCGCGGCCGCCGAAACATCTACTAAGCTTGAC-3′ Rev.: 5′ GATCCCCGGGGGATCC TCAGAAGGAAGTGAGCTCCGC-3′
(170-369)EgA31	Fwd.: 5′ ATGCGCGGCCGCCGCAGCT5GAAAAACAAGCCATG-3′ Rev.: 5′ GATCCCCGGGGGATCC TCACCTTGTTTCAAGCATTTCAAT-3′
(370-583)EgA31	Fwd.: 5′ ATGCGCGGCCGCCGCTGAGACTAAAGAAATTAAT-3′ Rev.: 5′ GATCCCCGGGGGATCC TCAATCTCTTTCGAGCTGTTTGAT-3′

The integrated restriction sites are underlined

The start and stop codons are label in bold