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. 2016 Aug 11;9:445. doi: 10.1186/s13071-016-1726-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Rhipicephalus microplus salivary inhibition of haemolysis by the classical and alternative pathways. A total volume of 125 μl was used for the haemolytic assay. Human serum was added to assess the effects of saliva on the process of haemolysis via the classical (2.1 μl) and alternative (12.5 μl) complement pathways, inducing at least 90 % of haemolysis. In (a) different amounts of saliva were tested and in (b) 7.5 μg of salivary proteins was used. Bars represent the arithmetic mean ± standard deviation (SD) from three biological replicates. Abbreviation; ns, non-significant (P > 0.05)