. 2016 Aug 11;9:171. doi: 10.1186/s13068-016-0581-3

Table 1.

Clusters of Orthologous Groups of proteins (COG) associated with the degradation of carbohydrates and proteins and with methanogenesis identified in the metagenome dataset of the thermophilic biogas microbiome

Environmental gene tag		Assigned sequences	Environmental gene tag		Assigned sequences
COG accession	Function	Assigned sequences	COG accession	Function	Assigned sequences
Polysaccharide degradation and metabolism			Methanogenesis—hydrogenotrophic pathway
COG0366	Glucosidase	12,524	COG1229	Formylmethanofuran dehydrogenase, subunit A	839
COG1472	Periplasmic beta-galactosidase and related glycosidases^a	11,683	COG2218	Formylmethanofuran dehydrogenase, subunit C	541
COG3459	Cellobiose phosphorylase	10,420	COG2191	Formylmethanofuran dehydrogenase, subunit E	371
COG1501	Alpha-glycosidase, GH31 family	5616	COG1153	Formylmethanofuran dehydrogenase, subunit D	212
COG2730	Aryl-phospho-beta-D-glycosidase BglC, GH1 family	2588	COG2037	Formylmethanofuran: H4MPT formyltransferase	269
COG1874	Beta-galactosidase GanA	1714	COG3252	Methenyl-H4MPT cyclohydrolase	227
COG2160	L-arabinose isomerase	1539	COG1962	H4MPT S-methyltransferase, subunit H	246
COG3345	Alpha-galactosidase	1385	COG4064	H4MPT S-methyltransferase, subunit G	181
COG1904	Glucuronate isomerase	1030	COG4059	H4MPT S-methyltransferase, subunit E	167
COG3325	Chitinase, GH18 family	958	COG4061	H4MPT S-methyltransferase, subunit C	151
COG3405	Endo-1,4-beta-D-glucanase Y^b	854	COG4060	H4MPT S-methyltransferase, subunit D	150
COG4124	Beta-mannanase^c	152	COG4063	H4MPT S-methyltransferase, subunit A	121
COG5297	Cellulase/cellobiase CelA1	136	COG4062	H4MPT S-methyltransferase, subunit B	14
COG0726	Peptidoglucan/xylan/chitin deacetylase, PgdA/CDA1 family	5831	COG4218	H4MPT S-methyltransferase, subunit F	8
COG3507	Beta-xylosidase	4780
GOG3693	Endo-1,4-beta-xylanase, GH 35 family	3797	Methanogenesis—acetoclastic pathway
GOG4213	ABC-type xylose transport system, periplasmic component	1628	COG1614	CO dehydrogenase/acetyl-CoA synthase, subunit β	510
GOG2115	Xylose isomerase	1591	COG1456	CO dehydrogenase/acetyl-CoA synthase, subunit γ (corrinoid Fe-S protein)	353
COG5434	Polygalacturonase	1023	COG2069	CO dehydrogenase/acetyl-CoA synthase, subunit δ (corrinoid Fe-S protein)	214
COG3867	Arabinogalactan endo-1,4-beta-galactosidase	823	COG1152	CO dehydrogenase/acetyl-CoA synthase, subunit α	198
COG3866	Pectate lyase	541	COG1880	CO dehydrogenase/acetyl-CoA synthase,subunit ε	55
COG4677	Pectin methylesterase and related acyl-CoA thioesterases	228
COG2132	Multicopper oxidase with three cupredoxin domains (includes cell division protein FtsP and spore coat protein CotA)	423	Methanogenesis—hydrogenotrophic and acetoclastic pathway
COG2132		423	COG4058	Methyl coenzyme-M reductase, subunit α	719
			COG4054	Methyl coenzyme-M reductase, subunit β	550
Protein degradation			COG4057	Methyl coenzyme-M reductase, subunit γ	270
COG0740	ATP-dependent protease ClpP, protease subunit	5178	COG4055	Methyl coenzyme-M reductase, subunit D	194
			COG4056	Methyl coenzyme-M reductase, subunit C	107

^aGH3 family

^bGH8 family

^cGH26 family