Logical Connectives |
! expr |
Is satisfied if expr is not satisfied. |
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expr | expr |
Is satisfied if at least one operand is satisfied. |
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expr ^ expr |
Is satisfied if at exactly one operand is satisfied. |
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expr & expr |
Is satisfied only if both operands are satisfied. |
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cond then then_expr else else_expr |
If cond is satisfied, this expression will only be satisfied if then_expr is satisfied. If cond is not satisfied, then this expression will only be satisfied if else_expression is satisfied. |
BAM Flags |
paired? |
The read is paired in sequencing. |
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proper_pair? |
The read is mapped in a proper pair |
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unmapped? |
The read is unmapped. |
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mate_unmapped? |
The mate is unmapped. |
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mapped_to_reverse? |
The read is mapped to the reverse strand. |
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mate_mapped_to_reverse? |
The mate is mapped to the reverse strand. |
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raw_flag(int) |
The read matches the specified SAM flag. |
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read1? |
The read is the first read in a pair. |
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read2? |
The read is the second read in a pair. |
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secondary? |
The alignment is not primary. |
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failed_qc? |
The read failed platform/vendor quality control checks. |
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duplicate? |
The read is either a PCR or optical duplicate |
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supplementary? |
The alignment is supplementary. |
Mapping Information |
chr(glob) |
The read is mapped to the specified chromosome. |
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mapping_quality(probability) |
Matches the read if the proability of error is less than the specified probability. |
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mate_chr(glob) |
The mate is mapped to the specified chromosome. |
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split_pair? |
Matches if both the read and it’s mate pair are mapped, but only if to different chromosomes. |
Position |
after(position) |
Matches all reads that cover the specified position or any higher position. |
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before(position) |
Matches all reads that cover the specified position or any lower position. |
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position(start,end) |
Matches all sequences that cover the range of position from specified start to end, i.e. N matches any base. |
Sequence |
nt(position,n) |
Matches all reads that are classified as the IUPAC-style nucleotide n at the specified position, i.e. N matches N. |
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nt_exact(position,n) |
Matches all reads that exactly match the IUPAC-style nucleotide n at the specified position. |
Miscellaneous |
read_group(glob) |
Matches the read group. |
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header ~ /regex/ |
Matches a Perl-compatible regular expression against the read’s header. |
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random(probability) |
The read is chosen based on a uniform pseudo-random variable. |
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true |
Always satisfied. |
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false |
Never satisfied. |