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. 2016 Aug 11;16:58. doi: 10.1186/s12896-016-0289-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Simplex and multiplex editing vector construction strategy. a & b Structure of simplex and multiplex editing binary vector based on pCAMBIA3301. P, promoter; T, terminator; Ubi P, ubiquitin promoter; NLS, nuclear localization sequence; Nos T, nopaline synthase terminator; BAR, phosphinothricin R; Kan R, kanamycin resistance gene; LB, left border; RB, right border; TGU, tRNA-gRNA unit