Fig. 4.

Phenotypic variations of simplex and multiplex editing mutants generated from the CRISPR/Cas9 targeting. a Comparison of zein protein contents from WT and MADS/CAS9 line 21 mature endosperm. The measurements were done on per 50 mg of dried endosperm. Error bars represent SD (n = 3) (***P < 0.001, Student’s t test). b SDS-PAGE detection of zein accumulation in WT and MADS/CAS9 line 21 mature endosperm. c Mature (35 DAP) WT and T0 transgenic maize plants of the mutant lines PPR/CAS9-9 and −13