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. 2016 Aug 12;6:82. doi: 10.3389/fcimb.2016.00082

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Copyright © 2016 Kuo, Chen, Chiu, Teng, Hsu, Lu, Syu, Wang, Chou and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mutations in rfaD reduce EHEC-induced intestinal microvillar actin rearrangement in C. elegans. (A) The confocal images showed the mCherry-tagged ACT-5 (intestinal microvillar actin) of GK454 transgenic animals fed with OP50, EDL933, EDL933 rfaD::Tn5 (rfaD::Tn5), EDL933:ΔrfaD (ΔrfaD) or EDL933:ΔrfaD-prfaD (ΔrfaD-prfaD) for 4 days respectively. Ectopic localization of the mCherry::ACT-5 signals from the apical membrane to the cytoplasm of the intestinal cells were significantly increased in the EDL933 treated group. Upper panels show mCherry images; lower panels are the merge images of the DIC and mCherry signals. Representative images are shown, and scale bar represents 10 μm. (B) Quantifications of animals with the EHEC-induced ectopic mCherry::ACT-5 signal were determined. Values represent the means of three independent assays, and error bars indicate the standard deviations. P-values denote the results of statistical analysis.