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. Author manuscript; available in PMC: 2016 Aug 12.
Published in final edited form as: Nat Protoc. 2015 May 28;10(6):927–938. doi: 10.1038/nprot.2015.054

Figure 3. Removal of unencapsulated small molecule from liposome preparations.

Figure 3

a) Repurification traces of vesicles (100 mM oleic acid) prepared with 1 mM calcein, dialyzed one (red trace), five (blue trace), or ten times (black trace) against a dye-free vesicle solution. b) Fraction of calcein encapsulated within vesicles after dialysis, calculated by integration of fluorescence signals from a), demonstrating removal of ca. 90% of free small molecules. Error bars indicate S.E.M., N=3.

The methods for preparation of fatty acid vesicles with encapsulated small molecule solutes and purification of vesicles are described in our previous work.1,21