FIG 3.

Protein purification and peptide pulldown analysis performed by students in the molecular CURE module. A) Example of student-generated SDS-PAGE and coomassie stain analysis of protein purification. The elution lane contains the purified GST-p300 bromodomain as indicated by the red arrow. B) Example of student-generated western blot results from peptide pulldown experiments performed with the indicated p300 bromodomain mutation. C) Validation of student generated results in the research laboratory setting showing negligent effects of the p300 bromodomain mutations on the acetyl-lysine binding function of the domain. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]