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. Author manuscript; available in PMC: 2016 Dec 10.
Published in final edited form as: Brain Res. 2015 Oct 21;1629:113–125. doi: 10.1016/j.brainres.2015.10.022

Figure 2.

Figure 2

Analysis of the growth-promoting effects on neurite outgrowth from DRG in early and late passage GRP. (A) Experimental design of co-culture and culture with conditioned medium. Immunostaining of DRG in control of DRG alone (B), co-culture with early (C-E) and late passage GRP (G-I), and in conditioned media from early (F) and late passage GRP (J). Scale bars = 150μm. Quantitative analysis of growth-promoting effects on neurite outgrowth from DRG in early and late passage GRP; (K) Primary neurite number, (L) Average neurite length, (M) Longest neurite length are shown in each graph. Asterisk represents significant differences relative to control (*P<0.05, **P<0.005). Pound sign represents significant differences relative to correspondents between co-culture and conditioned medium group (#P<0.05, ##P<0.005). Experiments were repeated three times and representative data are shown as mean ± standard error of the mean (SEM). Statistical analysis was performed by one-way ANOVA with Bonferroni test (N>70).