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. Author manuscript; available in PMC: 2017 Jul 21.
Published in final edited form as: Mol Cell. 2016 Jun 30;63(2):277–292. doi: 10.1016/j.molcel.2016.05.038

Figure 6. ΔNp63 depletion is associated with aberrant HME cell differentiation (Also see Figure S7).

Figure 6

6A) ΔNp63 is the primary p63 isoform in CD44low HME cells. The data represented are mean values±S.D. *** P<0.001

6B) ΔNP63α and ΔNP63β mRNA expression were significantly reduced in aberrantly differentiated CD44high cells after BRCA1 or BRG1 depletion or after Cisplatin treatment. The data represented are mean values±S.D. ***P<0.001.

6C-6E) Immunoblotting for ΔNp63 in CD44high cells after BRCA1/BRG1/FANCD2 depletion.

6F) Cisplatin suppresses endogenous ΔNP63 expression in CD44low HME cells.

6G-6I) ΔNP63 is necessary and sufficient to suppress the aberrant differentiation of CD44low cells. After siP63 transfection, the percentage of CD44high cells was measured, and expression of the EMT marker, Zeb1, and of the two HA-tagged ΔNP63 vectors was analyzed. ****P<0.0001.