Table 3.
List of genes involved in canonical pathways modulated significantly by SFN, 6-MSITC, and 6-MTITC in IMR-32 cells.
| ITCS | CANONICAL PATHWAYS | P-VALUE | REGULATION | NO. OF GENES | REGULATED GENES |
|---|---|---|---|---|---|
| SFN | Nrf2-mediated oxidative stress response | 5.01E-11 | ↑ | 12 | BACH1, DNAJB4, FTH1, FTL, GCLM, GSR, GSTM3, HMOX1, MAFF, NQO1, SQSTM1, TXNRD1 |
| TR/RXR activation | 6.03E-05 | ↑ | 5 | AKR1C1, AKR1C2, AKR1C3, ME1, SYT2 | |
| Metabolism of xenobiotics by cytochrome P450 | 8.71E-04 | ↑ | 4 | AKR1C1, AKR1C2, AKR1C3, GSTM3 | |
| Glutathione metabolism | 2.19E-03 | ↑ | 3 | GCLM, GSR, GSTM3 | |
| C21-steroid hormone metabolism | 3.72E-03 | – | 2 | AKR1C1, AKR1C3 | |
| 6-MSITC | Nrf2-mediated oxidative stress response | 1.23E-07 | ↑ | 14 | BACH1, DNAJB1, DNAJB4, FTH1, FTL, GCLM, GSR, HMOX1, KEAP1, MAFF, MAFG, NQO1, SQSTM1, TXNRD1 |
| TR/RXR activation | 1.12E-03 | ↑ | 6 | ACACA, AKR1C1, AKR1C2, AKR1C3, ME1, SYT2 | |
| Wnt/β-catenin signaling | 6.61E-03 | ↑ | 7 | GJA1, KREMEN1, PPP2R2B, PPP2R2C, SRC, WNT5A, WNT5B | |
| PTEN signaling | 8.91E-03 | ↑ | 5 | FOXO6, IKBKB, ITGA4, MAGI1, PDGFRA | |
| Ephrin receptor signaling | 1.05E-02 | ↑ | 7 | ANGPT1, CXCR4, FIGF, GNG12, ITGA4, NGEF, SRC | |
| 6-MTITC | Nrf2-mediated oxidative stress response | 4.68E-08 | ↑ | 14 | BACH1, DNAJB1, DNAJB4, FTH1, FTL, GCLM, GSR, HMOX1, KEAP1, MAFF, MAFG, NQO1, SQSTM1, TXNRD1 |
| TR/RXR activation | 1.23E-05 | ↑ | 8 | ACACA, AKR1C1, AKR1C2, AKR1C3, ME1, PPARGC1A, RXRA, SYT2 | |
| Hepatic Fibrosis/Hepatic stellate cell activation | 2.51E-04 | – | 8 | COL1A1, FGFR2, FIGF, FLT4, IGFBP5, PDGFRA, TNFRSF1A, VEGFA | |
| Axonal guidance signaling | 1.00E-03 | ↑ | 13 | CXCR4, FIGF, ITGA4, PLXNA2, PRKACB, SEMA6B, SHANK2, SHC1, SLIT2, SLIT3, VEGFA, WNT5A, WNT5B | |
| Estrogen receptor signaling | 3.24E-03 | ↑ | 6 | HNRNPD, PCK2, PPARGC1A, SHC1, SRC, TAF4B |