Table 4.
Effects of chronic administration of tryptophan and benserazide separately and in combination on concentrations of tryptophan and kynurenine metabolites in rat liver and serum.
| PARAMETER | LIVER | SERUM | ||||||
|---|---|---|---|---|---|---|---|---|
| CONTROL | BSZ | TRP | BSZ + TRP | CONTROL | BSZ | TRP | BSZ + TRP | |
| Trp | 12.3 ± 0.42 | 14.0 ± 0.7 | *26.7 ± 1.9 | *20.0 ± 1.7*¶ | 70 ± 3 | *58 ± 2 | *155 ± 9 | *96 ± 2*¶ |
| K | 7.3 ± 0.8 | *11.8 ± 1.0 | *11.8 ± 1.5 | *12.4 ± 1.4 | 3.3 ± 0.4 | 3.7 ± 0.4 | *6.8 ± 0.5 | 4.8 ± 0.6* |
| KA | 0.46 ± 0.14 | 0.58 ± 0.03 | *1.32 ± 0.28 | 0.42 ± 0.10* | 0.44 ± 0.16 | 0.24 ± 0.07 | *1.61 ± 0.32 | 0.36 ± 0.18* |
| AA | 1.99 ± 0.62 | 1.94 ± 0.50 | *4.39 ± 0.11 | 1.40 ± 0.33* | 2.59 ± 0.64 | 3.87 ± 0.37 | *7.83 ± 1.10 | 1.71 ± 0.22*¶ |
| 3-HK | 5.5 ± 1.5 | *52.6 ± 8.8 | *61.7 ± 9.2 | *112.5 ± 5.4*¶ | 8.8 ± 0.5 | *23.4 ± 1.0 | *28.5 ± 3.8 | *100.7 ± 5.7*¶ |
| XA | 0.17 ± 0.08 | 0.16 ± 0.02 | *1.50 ± 0.22 | *0.47 ± 0.04*¶ | 0.56 ± 0.07 | *0.09 ± 0.02 | 0.57 ± 0.07 | 0.58 ± 0.06¶ |
| 3-HAA | 2.43 ± 0.54 | 2.51 ± 0.70 | *7.42 ± 1.36 | 3.58 ± 0.66* | 0.01 ± 0.005 | 0.07 ± 0.04 | *6.79 ± 1.30 | *2.38 ± 1.03*¶ |
| KOHase | 88 ± 31 | *483 ± 111 | *564 ± 100 | *987 ± 114*¶ | 264 ± 50 | *626 ± 69 | 421 ± 82 | *2120 ± 199*¶ |
| KAT A | 4.5 ± 0.5 | 5.2 ± 0.6 | *9.3 ± 1.5 | 3.2 ± 0.5* | 13.2 ± 3.4 | 6.4 ± 2.6 | *26.0 ± 7.6 | 7.6 ± 4.6 |
| KAT B | 6.7 ± 1.4 | *0.3 ± 0.07 | *2.6 ± 0.5 | *0.4 ± 0.04* | 6.4 ± 0.9 | *0.4 ± 0.2 | *2.0 ± 0.3 | *0.6 ± 0.08* |
| Kynase A | 34 ± 8 | *18 ± 5 | 39 ± 3 | *12 ± 3* | 78 ± 34 | *17 ± 2 | 116 ± 19 | *36 ± 5* |
| Kynase B | 51.6 ± 6.6 | *5.6 ± 1.7 | *12.0 ± 1.3 | *3.0 ± 0.6* | 0.13 ± 0.07 | 0.30 ± 0.17 | *23.4 ±6.2 | 2.36 ± 1.05* |
| TDO | 59 ± 7 | *86 ± 10 | *44 ± 4 | 61 ± 3* | 4.8 ± 0.7 | 6.4 ± 0.9 | 4.4 ± 0.3 | 4.9 ± 0.5 |
| Ks | 3.0 ± 0.3 | *11.6 ± 1.4* | 16.0 ± 2.9 | *24.1 ± 1.4* | 2.6 ± 0.1 | *5.2 ± 0.2 | *8.7 ± 0.7 | *18.5 ± 0.9*¶ |
| TTOX | 24.4 ± 2.9 | *83.1 ± 8.5 | *59.3 ± 8.2 | *123.3 ± 10.4*¶ | 3.7 ± 0.3 | *9.0 ± 0.5 | 5.6 ± 0.7 | *19.2 ± 0.9*¶ |
Notes: Rats were treated intraperitoneally once daily for 8 days with saline, BSZ (100 mg/kg), Trp (50 mg/kg), or a combination of the latter two agents and were killed at 2 hours after the Trp injection on the final day. Saline and BSZ were given 1 hour or 15 minutes before Trp. Values in μM or ratio percentages are mean ± SEM for six rats per group. Asterisks on the left denote significant differences from controls, whereas those on the right denote significant differences between the Trp + BSZ group and the Trp group.
Denotes the differences between the combined group and the BSZ group. Symbols denote significant differences at P levels of 0.044–0.001.