Table 1.
Primers for methylation-specific polymerase chain reaction (PCR).
| Sequence | Length of product (bp) | |
|---|---|---|
| WIF-1 | ||
| MSP | F: 5′-GGTTTTATTGGGCGTATCGT-3′ | 145 |
| R: 5′-ACTAACGCGAACGAAATACGA-3′ | ||
| USP | F: 5′-GGGTGTTTTATTGGGTGTATTGT-3′ | 154 |
| R: 5′-AAAAAAACTAACACAAACAAAATACAAAC-3′ | ||
| RASSF1A | ||
| MSP | F: 5′-GTGTTAACGCGTTGCGTATC-3′ | 93 |
| R: 5′-AACCCCGCGAACTAAAAACGA-3′ | ||
| USP | F: 5′-TTTGGTTGGAGTGTGTTAATGTG-3′ | 105 |
| R: 5′-CAAACCCCACAAACTAAAAACAA-3′ | ||
| CDH13 | ||
| MSP | F: 5′-TGTATGAATGAAAACGTCGTC-3′ | 136 |
| R: 5′-GAATACAAAAACGAAACGCA-3′ | ||
| USP | F: 5′-GTGTATGAATGAAAATGTTGTT-3′ | 136 |
| R: 5′-CAAATACAAAAACAAAACACA-3′ | ||
MSP – methylation specific primer; USP – un-methylation specific primer; WIF-1 – Wnt inhibitory factor-1; RASSF1A – ras-association domain family member 1A; CDH13 – Cadherin 13.