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. 2016 Jun 22;107(8):1101–1109. doi: 10.1111/cas.12977

Figure 2.

Figure 2

Impact of contact‐free coculture with cells of the gastric cancer cell line OCUM‐12 on macrophage‐induced capillary structures of lymphatic endothelial cells (LECs). Phase contrast images are shown of contact coculture of LECs and macrophages in the absence (a,b) or presence (c,d) of contact‐free coculture with OCUM‐12 cells for 24 h (a,c) or 72 h (b,d). There was no morphological difference in LECs after 24 h of coculture; however, after 72 h, capillary morphogenesis of LECs was more apparent in the presence (d) than in the absence (b) of OCUM‐12 cells. Scale bar = 100 μm, all panels. (e) Median number of closed ring structures in the coculture was calculated and compared after 72 h with or without OCUM‐12 cells. There was a significant increase in the number of closed rings in the coculture with OCUM‐12 cells compared to the coculture without OCUM‐12 cells (P < 0.04). Statistical comparison was by the Wilcoxon rank sum test.